Senior Bacteriology Technician at Kikiktagruk Inupiat Corporation, LLC in Vienna, Virginia

Job Description:

Kikiktagruk Inupiat Corporation, LLC

Senior Bacteriology Technician
Closing Date: Until Filled
Department: Midnight Sun Professional Solutions
Location: Frederick, MD, USA
Employment Type: Full Time

Title: Senior Bacteriology Technician

Location: Frederick, MD

Status: Full Time

Travel: 10%

Salary Range: $80,000-$120,000

Position Summary:

USAMRIID, located at Fort Detrick, Maryland, works closely with other U.S. Army Medical Research and Development Command (USAMRDC) organizations, Department of Defense (DoD), the Interagency (including Interagency Laboratories), and private companies to accomplish its mission of protecting the Warfighter from biological threats and being prepared to investigate disease outbreaks or threats to public health. This includes conducting Research, Development, Testing, & Evaluation (RDTE) of medical countermeasure against biological threats and the safety, quality assurance, project management, and core support required for these research activities.

The Molecular Biology Division works to combat biological threats and weapons of mass destruction by developing high-throughput sequencing and protein profiling to identify emerging pathogens. It also evaluates and improves medical countermeasures, follows the DoD's roadmap for their development, and ensures their ethical use, including FDA approval. The Division also explores expanding indications and combinations with existing countermeasures.

The Automation And Assay Research Scientist shall provide Robotic Liquid Handling Automation support for research efforts of the TDC. Support will be required mainly in BSL-2 environment with subsequent potential support in BSL-3, and BSL-4 environments. Scientist will conduct basic and applied research on current and emerging threats in support of the BD and infectious disease program. They shall aid in the execution of scientific studies-throughput screening assays, enzymatic assays, collection/data analysis, maintain the laboratory and respective laboratory equipment. This position is contingent upon contract award.

Duties/Responsibilities:

• Assist PI in Molecular Microbiological Experiments aimed at defining elements and factors involved in bacterial virulence. Assist in approximately 2 animal protocols conducted by other investigators.
• Complete approximately 25 Opsonic Adherence assays to test the efficacy of animal sera (Rabbit and NHP) in the presence of Baby Rabbit Complement to adhere Ames bacilli and spores to RAW 264.7 macrophage cells.
• Complete over 1000 ELISAs (including retesting of samples where needed):
  • Yersinia pestis novel vaccine strategies
  • Burkholderia novel vaccine strategies
  • Yersinia pestis novel mAb identification
  • Burkholderia novel mAb identification
  • Francisella tularensis novel vaccine strategies/animal modeling
  • Coxiella burnetii novel vaccine strategies/animal modeling
• Prepare approximately 10 different Reagents / Media for the lab (including R Medium, DMEM/FBS, LB Broth, Sodium Bicarbonate, APS LB Agar, BHI, etc).
• Plate approximately 100 LB plates for use for Ames sporulation isolation and purification.
• Isolate and purify approximately 10 plasmid DNA specimens.
• FITC label and count approximately 10 Ames samples to check ratio of Bacilli: Spores over multiple time points.
• Order lab supplies for BSL-2 and BSL-3 laboratories including common supplies, custom orders, and equipment - approximately 400 items ordered along with 5 pieces of equipment.
• Present overview of data collected from Opsonic Adherence and ELISA at group meeting; present progress reports to supervising investigator. Use computer to prepare graphs and write reports for supervising investigator.
• Perform approximately 100 Immunological, antibody determinations.
• Perform approximately 100 ELISAs on mouse, nonhuman primate, and/or human serum.
• Perform approximately 50 Cytokine analyses. Measures cytokines in mouse, nonhuman primate, and/or human serum. Measures cytokines in cell cultures and cell extracts. Uses a Luminex instrument for analysis.
• Perform approximately 50 Flow Cytometry tasks, determining cellular distribution in mouse samples and intracellular cytokine expression. Uses a BD FACS Calibur and BD FACS Canto II to analyze cellular distribution.
• Perform approximately 25 Cell Biology/Biochemical/microbiological tasks. Performs laboratory tests, such as protein gel electrophoresis, western blot analysis, growth of macrophage cell lines, live-dead cell determinations, prepares microbiological media for bacterial cultures.
• Organize and maintain data generated from immunological and microbiological studies.
• Be responsible for managing approximately 80 hand receipt items and maintaining laboratory equipment.
• Complete approximately 10 anthrax toxin neutralization (TNA) assays per year.
• Perform approximately 15 animal challenges per year and approximately 10 bacterial challenge preparations to include planning and performing complex pathogenesis studies.
• Perform approximately 1,200 animal injections per year and approximately 2,500 animal observations per year.
• Complete approximately 500 bacterial burdens in organ tissue per year.
• Perform approximately 100 Luminex Cytokine Analyses per year.
• Perform approximately 75 Macrophage phagocytosis assays per year.
• Process approximately 200 ELISAs per year.
• Perform approximately 125 Animal Necropsies per year.
• Process approximately 150 animal gavage and 250 animal blood collections per year.
• Complete approximately 40 DNA chromosomal preparations, 500 PCR reactions; and 35 Agar gel analysis per year.
• Prepare approximately 20 cloning plasmids, approximately 50 sequencing reactions, and approximately 90 plasmid preparations.
• Complete approximately 100 media preparations per year.
• Perform approximately 250 BSL-3 live/dead assays per year.
• Process approximately 40 protein and western gels per year.
• Place approximately 50 GFEBs orders per year for supplies and equipment.
• Review relevant literature monthly.
• Serve as a training representative for BSL-2 laboratory skills.
• Assist PI with Project Planning, quarterly.
• Participate in training new personnel on laboratory tasks monthly.
• Perform BSAT Inventory Management weekly.
• Conduct Equipment Inventory Management twice a year in BSL-2 and BSL-3.
• Perform monthly Laboratory Management to include safety functions and chemical inventory.
• Assist the BSL-3 suite supervisor with suite supervisory duties.
• Interface with inspectors and perform monthly inspections.
• Be responsible for planning and performing animal experiments under BSL-3 conditions.
• Review relevant literature monthly. May assist in the preparation standard operating procedures and manuscripts relating to their research efforts. May be expected to present research findings at one scientific conference per year, which may require CONUS or OCONUS travel. Present results in written and oral format publicly and internally as requested.
• Provide 1 Bacteriology Technical Support monthly report to TPOC by the last working day of the month. Report would include materials and methods, data and data analysis and should be updated monthly.
• CS-PRP enrollment is required and shall be held and maintained IAW the provisions in this contract and all applicable regulations.
• BPRP enrollment is required and shall be held and maintained IAW the provisions in this contract and all applicable regulations.

Minimum Requirements:

• PhD or D.V.M with research experience in the fields of bacteriology, animal physiology, or similar field of research.
• Experience working under high containment (BSL-3 and ABSL-3) are preferred.
• 3+ years of research experience in the fields of bacteriology, animal physiology, or similar field.
• Prior experience designing, executing and analyzing all stages of complex studies including regulatory requirements associated with the FDA-animal rule.
• Must be able to obtain/maintain a Secret clearance.
• Active Secret clearance preferred.
• Must be eligible to enroll in the BPRP and CS-PRP programs
• Active BPRP and/or CS-PRP status preferred

Work Environment:

• Laboratory environment

Physical Demands:

• May lift up to 25lbs

Benefits:

KIC offers a comprehensive benefits package to eligible full-time employees including: Medical, Dental, and Vision Insurance, 401k with Match, Life and ADD Coverage, Short and Long Term Disability, Paid Time Off and Holidays, Education Assistance/Tuition Reimbursement, Employee Assistance Program, Employee Discount Program, and more!

Apply online at our website: https://kikiktagruk.applicantpool.com/

Disclaimer:

This is not to be an exclusive list of all responsibilities, duties, and skills required of the person in this job. KIC is an Equal Opportunity Employer. KIC considers all applicants for employment without regard to race, color, sex, national origin, religion, age, physical or mental disability, veteran's status or any other legal protected status.

Pursuant to The Alaska Native Claims Settlement Act 43 U.S.C. Sec. 1601 et seq., and federal contractual requirements, Kikiktagruk Inupiat Corporation, may legally grant certain preference in employment opportunities to KIC Shareholders, and their Descendants.

Successful candidates must adhere to KIC's Drug and Alcohol policy/testing requirements and may be required to pass a background and/or Motor Vehicle Records check.

The salary range for this position is based on several factors, including relevant experience, education, skills, and current market conditions.

Equal employment opportunity, including veterans and individuals with disabilities.

PI267351004

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