Working independently under the general supervision, executes protocols involving variety of routine and non-routine, highly specialized experimental procedures. Participates in the design and modification of research protocols under Laboratory Director Supervision. Will be working with research animals (mice and rats), cell culture, as well as animal tissue and blood specimens. Composes and presents sections of research reports, manuscripts or grant application. Performs advanced data analyses using advanced statistical techniques. Coordinates laboratory activities and undertakes quality assurance programs. Will be responsible for orienting and training staff. Will be able to analyze results on her/his own. Technician reports directly to Laboratory Director.
PRINCIPAL DUTIES AND RESPONSIBILITIES
Under the Supervision of Principal Investigator, designs and performs research laboratory experiments in the field of cardiovascular endocrinology, including:
a. Animal studies using rodent (rats and mice) colonies, including blood pressure measurements by tail cuff and telemetry.
b. Performs handling of rodents (e.g., mass, food consumption, urine collection)
c. Preparation and maintenance of cell cultures.
d. Preparation and analysis of DNA, RNA, and mRNA
e. Isolation and analysis of proteins including electrophoresis, transfections, etc.
f. Isolate and collect tissues relevant to the phenotype being studied (e.g. heart, kidneys, conductance/resistance arteries, adrenal glands, etc.).
g. Separate Zona Glomerulosa and Zona Fasciculata cells from the collected adrenals, and perform adrenal cell stimulation experiments.
h. Measure analytes in animal serum/plasma (e.g. hormones, cations, etc.)
Record data, analyzes and summarizes results. Utilizes computer spread sheets and graphics to prepare data plots and figures.
Prepares and maintain laboratory reagents, chemicals, instruments, and equipment.
Maintain supplies and inventory, and maintain established departmental policies, procedures, objectives, quality assurance, safety, environmental and infection control.
Assist in the training of new lab members.
Discusses program, regularly with Principal Investigator.
Performs other duties and responsibilities as directed.
WORKING CONDITIONS
Normal laboratory environments.
May have exposure to infection materials and hazardous substances, exposure to odors, exposure to noise, under controlled conditions.
Possible visual strain and hand strain encountered while performing specimen-processing or while using computer terminals.
Work requires frequent standing, walking and occasional lifting or carrying supplies.
Qualifications
QUALIFICATIONS
BS in biological/physical science.
SKILLS/ ABILITIES/ COMPETENCIES REQUIRED
Computer skills (intermediate level) including word processing and spreadsheet applications required.
Must be extremely organized and capable of working under pressure.
Must be proficient in basic laboratory procedures: Westerns, RT-PCR, immunoassays.
Be flexible to adjust schedule in accordance with the needs of the department.
Ability to identify potential or on-going problems and help develop solutions to such problems.
Must have the ability to work independently.
Must possess initiative.
Must possess good interpersonal skills to interact courteously and effectively with co-workers, research investigators, other hospital personnel, and external contacts.
Must be able to work with animals - rodents.
Must show a desire to learn new techniques, disease-related approaches, etc.
Experience performing surgeries in animals is a plus.
EEO Statement
BWH is an Affirmative Action Employer. By embracing diverse skills, perspectives and ideas, we choose to lead. All qualified applicants will receive consideration for employment without regard to race, color, religious creed, national origin, sex, age, gender identity, disability, sexual orientation, military service, genetic information, and/or other status protected under law. We will ensure that all individuals with a disability are provided a reasonable accommodation to participate in the job application or interview process, to perform essential job functions, and to receive other benefits and privileges of employment.
