Posted in Other 12 days ago.
Type: Full-Time
Location: Pittsburgh, Pennsylvania
The Roth Lab is seeking someone motivated for the position of Wet Laboratory Research Technician III. The successful candidate will be an integral part of a dynamic, interdisciplinary team of researchers, graduate students, and postdocs, investigating the function and impact of human DNA sequence variation. The Wet Laboratory Research Technician III will work closely with students and staff in the lab to assist in the prioritization of experiments. The Wet Laboratory Research Technician III will play an important role facilitating the day-to-day operations of the lab and will be required to perform moderately complex research activities
Performs a variety of independent laboratory work and experiments such as determining experimental protocols and preparation of specimens and cultures. Prepares reports of findings subsequent to compilation and analyzation. Oversees specimen inventory. Develops documentation for publication; crafts manuscripts and grant proposals.
Responsibilities: Experimentation using human cell culture (routine handling, transfection, flow cytometry). Manipulating and screening large libraries of bacterial and yeast cells. DNA isolation from cells, PCR amplification, and gel purification of libraries. Preparing and processing of DNA libraries for sequencing using high throughput sequencing platforms (e.g., Illumina and PacBio). Operating liquid- and plate-handing robotics. Maintaining laboratory stocks of media supplies, reagents, buffers, plates, etc. Performing routine maintenance of laboratory instruments. Assisting in analyzing and summarizing data for scientific communications (e.g., grant applications, peer-reviewed publications). Maintaining records of experimental data and communicating results to lab members. Reviewing pertinent scientific literature.
Physical effort associated with wet lab work, i.e. standing while conducting experiments, sitting while working on computer, walking back and forth in the lab to sinks and microscopes, lifting packages less than 20lbs., etc.
Qualifications: Theoretical and practical knowledge of molecular cloning techniques is essential (DNA/RNA isolation, restriction digestion, PCR amplification, purification, etc.). Experience with human cell culture OR the handing/manipulation of yeast is essential. Experience with the proper handling and genetic manipulation of bacteria is essential. Proficiency with basic computational tools used in biology (Excel, BLAST, primer design software) is essential. Experience with the preparation and quality control of Illumina sequencing libraries would be an asset. Experience with flow cytometry and FACS would be an asset.
The University of Pittsburgh is committed to championing all aspects of diversity, equity, inclusion, and accessibility within our community. This commitment is a fundamental value of the University and is crucial in helping us advance our mission, which includes attracting and retaining diverse workforces. We will continue to create and maintain an environment that allows individuals to discover, belong, contribute, and grow, while honoring the experiences, perspectives, and unique identities of all.
The University of Pittsburgh is an Affirmative Action/Equal Opportunity Employer and values equality of opportunity, human dignity and diversity. EOE, including disability/vets.
PI254885904
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