Job Description:

Research Technician

The Department of Structural Biology at the University of Pittsburgh School of Medicine is located in Biomedical Science Tower 3. We currently have twelve faculty members working in the fields of cryo-electron microscopy, NMR spectroscopy, X-ray crystallography, and biochemistry. This laboratory technician position requires a B.S. degree plus at least one year of experience in protein expression and purification and related skills.

Job Summary

Performs a variety of laboratory experiments and procedures. Prepares specimens. Oversees supply management. Operates and properly maintains equipment.

Essential Functions

Conducting and managing laboratory experiments. Manage multiple research projects concurrently using techniques such as PCR, DNA cloning, sample preparation for electron microscopy, and cryo-EM data collection/analysis. Work together with PI or independently to perform complex laboratory work and technical analysis. Troubleshoot technical problems and find suitable experimental solutions or design new procedures to circumvent problems.

The applicant should be highly motivated, well-organized, detail-oriented, be able to prioritize multiple tasks, and will be encouraged to work independently. Strong interpersonal skills and judgment is essential. The successful applicant should have some experience in molecular biology including PCR, cloning, SDS-PAGE, fluorescent imaging, and cell culture. Experience in cryo-EM (electron microscopy, FIB milling) and proficient computer skills (Linux, python, scripting) are highly desired but not essential. Most of all we are looking for applicants that are motivated to learn novel technologies, take on new challenges, and to develop innovative techniques and methods.

The successful candidate will work with other lab members to conduct cell culture, molecular and cellular biology (cloning, genetic manipulation of singe-cell system), immunofluorescence-labelling, and cryo-electron tomography. Organization of lab space, coordination with other lab members for ordering and stocks, and presentation of data at lab meetings are essential. The candidate will assist the PI with experimental design, implementation of novel strategies/procedures, and manuscript preparation for publication, including figures and writing.

Physical Effort

Ability to manipulate laboratory instruments and reagents to conduct experiments; ability to move about the lab environment. Will require standing or sitting at lab benches for long periods of time.

The University of Pittsburgh is committed to championing all aspects of diversity, equity, inclusion, and accessibility within our community. This commitment is a fundamental value of the University and is crucial in helping us advance our mission, which includes attracting and retaining diverse workforces. We will continue to create and maintain an environment that allows individuals to discover, belong, contribute, and grow, while honoring the experiences, perspectives, and unique identities of all.

The University of Pittsburgh is an Affirmative Action/Equal Opportunity Employer and values equality of opportunity, human dignity and diversity. EOE, including disability/vets.

Assignment Category: Full-time regular

Job Classification: Staff.Laboratory Research Technician II

Job Family: Research

Job Sub Family: Laboratory

Campus: Pittsburgh

Minimum Education Level Required: Bachelor's Degree

Minimum Years of Experience Required: 1

Will this position accept substitution in lieu of education or experience: Combination of education and relevant experience will be considered in lieu of education and/ or experience requirement.

Work Schedule: Monday - Friday, 8:30 a.m. - 5:00 p.m.

Work Arrangement: Monday - Friday, 8:30 a.m. - 5:00 p.m.

Hiring Range: To be determined based on qualifications

Relocation_Offered: No

Visa Sponsorship Provided: Yes

Background Check: For position finalists, employment with the University will require successful completion of a background check

Child Protection Clearances: Not Applicable

Required Documents: Resume

Optional Documents: Cover Letter

PI254206348

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